cell dye bodipy 493 503  (MedChemExpress)

Journal: Nature Communications

Article Title: Diet-induced RKIP downregulation disrupts PC/PE-ER homeostasis to drive MASLD

doi: 10.1038/s41467-025-65982-8

Figure Lengend Snippet: a Representative fluorescence images of Bodipy 493/503-stained LDs (left), the percentage of LDs (middle; Fresh groups, n = 6; Veh/PA groups, n = 5) and the mean number of LDs per cell (right, n = 20) in primary hepatocytes from 2 months old RKIP f/f and Alb cre RKIP f/f mice (Fresh cells without plating (Fresh); cells plating 24 hrs with or without PA treatment). Green, Bodipy 493/503; Blue, DAPI; Scale bars, 40 μm. b Mean fluorescent intensity (MFI) of Bodipy 493/503 in mouse primary hepatocytes as described in A ( n = 3), determined by flow cytometry. c, e Representative fluorescence images of LDs, the percentage of LDs (HepG2 groups, n = 3; HeLa groups, n = 5) and the mean number of LDs per cell ( n = 20) in WT and RKIP knockout (KO) HepG2 ( c ) and HeLa cells ( e ) with PA stimulation for 24 hrs. Scale bars, 40 μm. d , f MFI of Bodipy 493/503 in PA-stimulated WT and KO HepG2 ( d ) and HeLa cells ( f ) ( n = 3), determined by flow cytometry. g, h Representative fluorescence images of LDs (left), the percentage of LDs (middle; n = 4) and the mean number of LDs per cell (right, n = 20) in PA (24 hrs) -stimulated WT and KO HepG2 ( g ) and HeLa ( h ) cells transduced with Vector or RKIP-flag lentivirus. Scale bars, 40 μm. Data are presented as mean ± SEM ( a–h ). Each dot represents a technical replicate ( a, c, e, g, h ) or a biological replicate ( b, d, f ). ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001. P values were calculated by two-tailed unpaired Student’s t -tests ( a–f ) and one-way ANOVA in ( g, h ). Data are representative of at least two independent experiments. Source data are provided as a Source Data file .

Article Snippet: Golgi was stained by living cell dye Golgi-tracker (Beyotime Biotechnology, C1043) at 4 °C for 30 min. LDs were stained by living cell dye Bodipy 493/503 (4 mM, MedChemExpress, HY-W090090).

Techniques: Fluorescence, Staining, Flow Cytometry, Knock-Out, Transduction, Plasmid Preparation, Two Tailed Test

Journal: Cell Death Discovery

Article Title: Perilipin2-dependent lipid droplets accumulation promotes metastasis of oral squamous cell carcinoma via epithelial-mesenchymal transition

doi: 10.1038/s41420-025-02314-1

Figure Lengend Snippet: A GO and KEGG analysis showed that the differentially expressed genes after PA stimulation were enriched in diverse aspects closely related to tumor metastasis like lipid metabolism, adhesion, and angiogenesis. B qRT-PCR and WB results showed that the expression level of PLIN2 in both HSC3 and HSC6 cells was up-regulated after 25 μM PA stimulation. C BODIPY staining results showed that 25 μM PA stimulation enhanced LDs accumulation in different OSCC cells. D qRT-PCR and WB results confirmed that the expression levels of PLIN2 in HSC3 and CAL33 cells were significantly increased after stable infection with lentiviral vectors. E BODIPY staining results showed that overexpression of PLIN2 enhanced LDs accumulation in HSC3 cells. F qRT-PCR and WB results confirmed that the expression level of PLIN2 in HSC6 cells was significantly decreased after transient transfection with Si-RNA. G BODIPY staining results showed that knockdown of PLIN2 reduced the accumulation of LDs in HSC6 cells, and the promoting effect of 25 μM PA stimulation on intracellular LDs accumulation was also decreased correspondingly. Bar: 10 μm. The green fluorescence in ( C , E , G ) is the BODIPY staining of LDs, and the blue fluorescence is the Hoechst staining of the nucleus. * P < 0.05, ** P < 0.01, *** P < 0.001. FAs Fatty acids, PA Palmitic acid, LDs Lipid droplets, NC Negative control.

Article Snippet: Intracellular LDs were measured using the cell permeable fluorescent lipophilic dye, BODIPY 493/503 (Thermo Fisher Scientific, USA).

Techniques: Quantitative RT-PCR, Expressing, Staining, Infection, Over Expression, Transfection, Knockdown, Fluorescence, Negative Control

Journal: Cell Death Discovery

Article Title: Perilipin2-dependent lipid droplets accumulation promotes metastasis of oral squamous cell carcinoma via epithelial-mesenchymal transition

doi: 10.1038/s41420-025-02314-1

Figure Lengend Snippet: A Transwell results showed that overexpression of PLIN2 can increase the in vitro invasion ability of HSC3 and CAL33 cells. Bar: 50 μm. B Transwell results showed that knockdown of PLIN2 decreased the promoting effect of 25 μM PA stimulation on the in vitro invasion of HSC6 cells. Bar: 50 μm. C qRT-PCR results confirmed that after transient transfection with Si-RNA, the expression level of DGAT1 in HSC3 cells stably overexpressing PLIN2 was significantly reduced. D BODIPY staining results showed that knockdown of DGAT1 reduced LDs generation in HSC3 cells stably overexpressing PLIN2. Bar: 10 μm. The green fluorescence in this figure is the BODIPY staining of LDs, and the blue fluorescence is the Hoechst staining of the nucleus. E Transwell results indicated that knockdown of DGAT1 significantly decreased the in vitro invasion ability of HSC3 cells stably overexpressing PLIN2. Bar: 50 μm. * P < 0.05, ** P < 0.01, *** P < 0.001. PA: Palmitic acid; NC: Negative control; LDs: Lipid droplets.

Article Snippet: Intracellular LDs were measured using the cell permeable fluorescent lipophilic dye, BODIPY 493/503 (Thermo Fisher Scientific, USA).

Techniques: Over Expression, In Vitro, Knockdown, Quantitative RT-PCR, Transfection, Expressing, Stable Transfection, Staining, Fluorescence, Negative Control